The RNF20 Polyclonal Antibody (CAB17309) is a valuable tool for researchers studying the RNF20 protein, which plays a key role in regulating gene expression and chromatin structure. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.RNF20 is a ubiquitin ligase that monoubiquitinates histone H2B, leading to the regulation of gene transcription and chromatin remodeling. Dysregulation of RNF20 has been implicated in various diseases, including cancer, making it a potential therapeutic target for drug development.
By using the RNF20 Polyclonal Antibody, researchers can detect and analyze the expression of RNF20 in different cell types, allowing for a better understanding of its role in gene regulation and chromatin dynamics. This antibody is an essential tool for studies in molecular biology, epigenetics, and cancer research, where RNF20 is a key player in controlling cellular processes.
Product Name:
RNF20 Rabbit Polyclonal Antibody
SKU:
CAB17309
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNF20 (NP_062538.5).
The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS).
Purification Method:
Affinity purification
Gene ID:
56254
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RNF20 Rabbit pAb (CAB17309) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
