The RNF2 Polyclonal Antibody (CAB5563) is specifically designed for research involving RNF2, a key protein involved in the regulation of gene expression and cell differentiation. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the RNF2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and developmental biology.RNF2, also known as Ring1B, is a critical component of polycomb repressive complex 1 (PRC1) and plays a crucial role in epigenetic regulation and cell fate determination.
Its functions in gene silencing and chromatin remodeling make it a potential target for research into various diseases, including cancer and developmental disorders. Understanding the mechanisms by which RNF2 regulates gene expression is essential for advancing our knowledge of cellular processes and disease pathology. The RNF2 Polyclonal Antibody provides researchers with a valuable tool for investigating the functions of RNF2 and its implications in health and disease.
Product Name:
RING1B/RNF2 Rabbit Polyclonal Antibody
SKU:
CAB5563
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RING1B/RING1B/RNF2 (NP_009143.1).
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.
Purification Method:
Affinity purification
Gene ID:
6045
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using RING1B/RNF2 Rabbit pAb (CAB5563) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
