The RNF185 Polyclonal Antibody (CAB16158) is a valuable tool for researchers studying the RNF185 protein, a key player in the regulation of protein turnover and cell signaling processes. This antibody, generated in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for use in Western blot applications.RNF185, also known as RING finger protein 185, is involved in the ubiquitin-proteasome pathway, where it plays a critical role in tagging proteins for degradation and maintaining cellular homeostasis. Dysregulation of RNF185 has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular health and disease progression.
By using the RNF185 Polyclonal Antibody, researchers can effectively study the expression levels and localization of RNF185 in different cell types and tissues, allowing for a better understanding of its function and potential therapeutic implications. This antibody is a valuable tool for investigations in molecular biology, cell biology, and disease pathology, providing crucial insights into the role of RNF185 in physiological and pathological processes.
Product Name:
RNF185 Rabbit Polyclonal Antibody
SKU:
CAB16158
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RNF185 (NP_689480.2).
Endoplasmic reticulum membrane, Mitochondrion outer membrane, Multi-pass membrane protein
Calculated MW:
20kDa
Observed MW:
20kDa
Enables ubiquitin binding activity; ubiquitin protein ligase activity; and ubiquitin-like protein conjugating enzyme binding activity. Involved in positive regulation of ERAD pathway; protein autoubiquitination; and ubiquitin-dependent ERAD pathway. Located in endoplasmic reticulum.
Purification Method:
Affinity purification
Gene ID:
91445
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse testis, using RNF185 Rabbit pAb (CAB16158) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
