The RNF138 Polyclonal Antibody (PAC022321) is a valuable tool for researchers studying the role of RNF138 in cellular processes and protein regulation. Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the RNF138 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in molecular biology and cell signaling pathways.RNF138, a RING finger protein, is known for its involvement in regulating protein stability and turnover through the ubiquitin-proteasome pathway. Its function in protein degradation makes it a key player in maintaining cellular homeostasis and responding to cellular stress.
Studies on RNF138 can provide insights into mechanisms of protein regulation and potential therapeutic targets for diseases related to protein misfolding and aggregation.By utilizing the RNF138 Polyclonal Antibody, researchers can deepen their understanding of the role of RNF138 in cellular processes and develop new avenues for exploring its potential as a target for therapeutic intervention. This antibody is a valuable tool for studies in areas such as protein degradation, cellular signaling, and disease mechanisms.
Antibody Name:
RNF138 Antibody (PACO22321)
Antibody SKU:
PACO22321
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human RNF138.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from MCF-7 cells, using RNF138 antibody.
Background:
E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.
Synonyms:
E3 ubiquitin-protein ligase RNF138; EC 6.3.2.-; RING finger protein 138; Nemo-like kinase-associated RING finger protein; NLK-associated RING finger protein
UniProt Protein Function:
RNF138: E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.19Chromosomal Location of Human Ortholog: 18q12.1Cellular Component: nucleusMolecular Function: protein binding; protein kinase binding; single-stranded DNA binding; ubiquitin conjugating enzyme bindingBiological Process: double-strand break repair via homologous recombination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
