The RNF111 Polyclonal Antibody (CAB18277) is a high-quality antibody designed for research involving RNF111, a key regulator of DNA damage response and repair processes. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the RNF111 protein, this antibody enables accurate detection and analysis in various cell types, making it well-suited for studies in the fields of DNA repair, genomic stability, and cancer research.
RNF111, also known as Arkadia, plays a crucial role in maintaining genome integrity by promoting the repair of DNA damage and regulating cell cycle progression. Dysregulation of RNF111 has been implicated in various human diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular homeostasis. By understanding the function of RNF111, researchers can develop targeted therapies for diseases associated with genomic instability and DNA damage.
Product Name:
RNF111 Rabbit Polyclonal Antibody
SKU:
CAB18277
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human RNF111 (NP_001257459.1).
cytoplasm, cytosol, nucleoplasm, nucleus, PML body
Calculated MW:
109kDa
Observed MW:
120kDa
The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
54778
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RNF111 Rabbit pAb (CAB18277) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
