The RNASEH2B Polyclonal Antibody (CAB4972) is a valuable tool for researchers studying RNASEH2B, an essential component of the ribonuclease H2 enzyme complex involved in DNA replication and repair. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the RNASEH2B protein, this antibody enables reliable detection and analysis in a variety of cell types, making it suitable for investigations in genetics, molecular biology, and cancer research.
RNASEH2B is critical for maintaining genomic stability through its role in removing RNA primers during DNA replication. Dysregulation of RNASEH2B has been implicated in various genetic disorders and cancer development, underscoring the importance of studying its functions and mechanisms. By utilizing the RNASEH2B Polyclonal Antibody, researchers can gain insights into the biochemical properties and cellular functions of RNASEH2B, ultimately advancing our understanding of DNA replication and repair processes.
Product Name:
RNASEH2B Rabbit Polyclonal Antibody
SKU:
CAB4972
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human RNASEH2B (NP_078846.2).
RNase H2 is composed of a single catalytic subunit (A) and two non-catalytic subunits (B and C) and specifically degrades the RNA of RNA:DNA hybrids. The protein encoded by this gene is the non-catalytic B subunit of RNase H2, which is thought to play a role in DNA replication. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Aicardi-Goutieres syndrome type 2 (AGS2).
Purification Method:
Affinity purification
Gene ID:
79621
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RNASEH2B Rabbit pAb (CAB4972) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
