The RIPK1 Polyclonal Antibody (CAB7414) is a valuable tool for researchers studying receptor-interacting serine/threonine-protein kinase 1 (RIPK1), a key player in cell death and inflammation pathways. Raised in rabbits, this antibody demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the RIPK1 protein, researchers can accurately detect and analyze its presence in a variety of cell types.RIPK1 is known for its critical role in regulating cell death pathways, particularly in response to stress or damage.
Dysregulation of RIPK1 has been implicated in various diseases, such as inflammatory disorders, neurodegenerative diseases, and cancer. Thus, understanding the function and regulation of RIPK1 is essential for developing targeted therapies for these conditions.The RIPK1 Polyclonal Antibody is a valuable tool for researchers interested in unraveling the complex mechanisms involving RIPK1 and its implications in disease pathology. Its high specificity and reactivity with human samples make it an ideal choice for studies in immunology, cell biology, and cancer research.
Product Name:
RIPK1/RIP Rabbit Polyclonal Antibody
SKU:
CAB7414
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RIPK1/RIP (NP_003795.2).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
RIP; RIP1; AIEFL; IMD57; RIP-1; RIPK1/RIP
Positive Sample:
Raji,Jurkat,Mouse liver,C6
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm.
Calculated MW:
76kDa
Observed MW:
75kDa/38kDa
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth.
Purification Method:
Affinity purification
Gene ID:
8737
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using RIPK1/RIP Rabbit pAb (CAB7414) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
