The RNF7 Antibody (CAB7301) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in various applications, including Western blotting.RNF7 is known to be involved in the regulation of protein degradation, specifically targeting proteins for ubiquitination and subsequent degradation by the proteasome. This process plays a crucial role in maintaining cellular homeostasis and is implicated in various cellular processes, including cell cycle regulation, signal transduction, and DNA repair.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
RNF7 Antibody
SKU:
CAB7301
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SAG, ROC2, rbx2, CKBBP1, RNF7
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
13kDa
Observed MW:
Refertofigures
The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Purification Method
Affinity purification
Gene ID
9616
RRID
AB_2767842
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Immunofluorescence analysis of MCF-7 cells using RNF7 Rabbit pAb (CAB7301). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
