The RIN3 Antibody (PACO58869) is a polyclonal antibody specifically designed for research involving RIN3, a crucial intracellular protein involved in regulating endocytic trafficking and cellular signaling processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.RIN3 plays a critical role in the regulation of cellular processes such as receptor internalization, vesicle trafficking, and signal transduction. Its involvement in these pathways makes it a key target for studying various physiological and pathological conditions, including cancer, neurodegenerative disorders, and infectious diseases.
By detecting and analyzing RIN3 protein expression in different cell types, this antibody facilitates the investigation of the mechanisms underlying these diseases.Understanding the function of RIN3 is essential for developing potential therapeutic strategies that target its activity, offering new possibilities for treating disorders linked to aberrant endocytic and signaling pathways. Researchers can utilize this antibody to gain insights into the role of RIN3 in cellular physiology and disease pathogenesis, advancing our understanding of these complex biological processes.
Antibody Name:
RIN3 Antibody (PACO58869)
Antibody SKU:
PACO58869
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Ras and Rab interactor 3 protein (245-465AA)
Immunofluorescence staining of Hela cells with PACO58869 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Ras effector protein that functions as a guanine nucleotide exchange (GEF) for RAB5B and RAB31, by exchanging bound GDP for free GTP. Required for normal RAB31 function.
Synonyms:
Ras and Rab interactor 3 (Ras interaction/interference protein 3), RIN3
UniProt Protein Function:
Ras effector protein that functions as a guanine nucleotide exchange (GEF) for RAB5B and RAB31, by exchanging bound GDP for free GTP. Required for normal RAB31 function.
UniProt Protein Details:
NCBI Summary:
Summary: This protein encoded by this gene is a member of the RIN family of Ras interaction-interference proteins, which are binding partners to the RAB5 small GTPases. The protein functions as a guanine nucleotide exchange for RAB5B and RAB31. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
