The RIG-I/DDX58 Monoclonal Antibody (CAB22478) is a crucial tool for research involving the RIG-I/DDX58 protein, a key player in the innate immune response to viral infections. This monoclonal antibody, produced through hybridoma technology, is highly specific to human samples and has been validated for use in various applications, including immunofluorescence and flow cytometry.RIG-I/DDX58 is a cytosolic RNA helicase that recognizes viral RNA and triggers the production of type I interferons, leading to the activation of antiviral immune responses. Dysfunction in the RIG-I/DDX58 pathway has been linked to increased susceptibility to viral infections and the development of autoimmune diseases.
This makes the RIG-I/DDX58 Monoclonal Antibody an invaluable tool for studying the mechanisms of viral recognition and immune response activation.Researchers in virology, immunology, and infectious diseases will find the RIG-I/DDX58 Monoclonal Antibody essential for investigating the role of RIG-I/DDX58 in viral pathogenesis and host defense mechanisms. Its high specificity and reliability make it a trusted reagent for understanding the intricate interplay between viruses and the innate immune system. Unlock the secrets of viral recognition and immune activation with the RIG-I/DDX58 Monoclonal Antibody (CAB22478).
Product Name:
Rig-I/DDX58 Monoclonal Antibody
SKU:
CAB22478
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 241-455 of human Rig-I/DDX58 (NP_055129.2).
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.
Purification Method:
Affinity purification
Gene ID:
23586
Clone Number:
ARC54110
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Rig-I/DDX58 antibody (CAB22478) at 1:2000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
