The Ribonuclease H1 Polyclonal Antibody (CAB9116) is a valuable tool for researchers studying Ribonuclease H1, an enzyme involved in the degradation of RNA-DNA hybrids. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to Ribonuclease H1, this antibody allows for the detection and analysis of the enzyme in various cell types, making it ideal for investigations in molecular biology and RNA research.Ribonuclease H1 plays a crucial role in the processing of RNA molecules within cells, making it a key player in gene expression regulation. Its function in RNA degradation and RNA-DNA hybrid resolution has implications for diverse biological processes, including DNA replication, RNA maturation, and viral infection.
Understanding the activity of Ribonuclease H1 is essential for unraveling these complex mechanisms and developing targeted therapies for RNA-related diseases.Researchers interested in exploring the intricacies of RNA metabolism, gene regulation, and viral replication will find the Ribonuclease H1 Polyclonal Antibody (CAB9116) to be a valuable asset in their experimental toolkit. Its specificity and reliability make it a valuable resource for advancing our understanding of cellular processes involving RNA-DNA hybrids and RNA degradation.
Product Name:
RNASEH1 Rabbit Polyclonal Antibody
SKU:
CAB9116
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human RNASEH1 (NP_002927.2).
This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17.
Purification Method:
Affinity purification
Gene ID:
246243
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RNASEH1 antibody (CAB9116) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
