The Rhou Polyclonal Antibody (PAC020925) is a valuable tool for researchers studying the Rhou protein, a key regulator of cell migration and cytoskeletal dynamics. This antibody, produced in rabbits, has high specificity for human samples and is optimized for use in Western blotting applications. By targeting the Rhou protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an ideal choice for studies in cell biology, cancer metastasis, and signaling pathways involved in cell movement.
Rhou, a small GTPase protein, plays a critical role in coordinating actin cytoskeleton dynamics and cell migration processes. Its involvement in these cellular functions makes it a promising target for research on cancer progression, metastasis, and other diseases characterized by aberrant cell motility. Understanding the intricate mechanisms of Rhou activity is essential for developing targeted therapies that can potentially disrupt or enhance cell migration in a controlled manner.
Antibody Name:
RHOU Antibody (PACO20925)
Antibody SKU:
PACO20925
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:20-1:100
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human RHOU
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO20925(RHOU Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO20925(RHOU Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.
Synonyms:
ras homolog family member U
UniProt Protein Function:
Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.
NCBI Summary:
This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.[provided by RefSeq, Mar 2011]
