RhoC Polyclonal Antibody (CAB21798)
- SKU:
- CAB21798
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IF
Frequently bought together:
Description
| Product Name: | RhoC Polyclonal Antibody |
| SKU: | CAB21798 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 94-193 of human RhoC (NP_786886.1). |
| Sequence: | NIPE KWTP EVKH FCPN VPII LVGN KKDL RQDE HTRR ELAK MKQE PVRS EEGR DMAN RISA FGYL ECSA KTKE GVRE VFEM ATRA GLQV RKNK RRRG CPIL |
| Tested Applications: | WB IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 |
| Synonyms: | H9; ARH9; ARHC; RHOH9; RhoC |
| Positive Sample: | Rat heart |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cell membrane, Cleavage furrow, Cytoplasmic side, Lipid-anchor. |
| Calculated MW: | 22kDa |
| Observed MW: | 22kDa |
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
| Purification Method: | Affinity purification |
| Gene ID: | 389 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Rat heart, using RhoC Rabbit pAb (CAB21798) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. |
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