The RhoB Polyclonal Antibody (CAB16762) is a valuable tool for researchers studying RhoB, a small GTPase protein involved in cellular signaling and regulation. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It binds specifically to the RhoB protein, allowing for precise detection and analysis in various cell types.RhoB is known to play a critical role in cellular processes such as cell migration, apoptosis, and cytoskeletal organization. Dysregulation of RhoB has been implicated in diseases such as cancer, cardiovascular disorders, and neurological conditions.
Therefore, studying the expression and activity of RhoB is essential for understanding its impact on cellular function and disease progression.By utilizing the RhoB Polyclonal Antibody, researchers can investigate the role of RhoB in various biological processes and diseases. This antibody enables accurate detection and analysis of RhoB protein levels, providing valuable insights into the molecular mechanisms underlying its function. Overall, the RhoB Polyclonal Antibody is a valuable tool for advancing research in cell biology, cancer biology, and other related fields.
Product Name:
RhoB Rabbit Polyclonal Antibody
SKU:
CAB16762
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-196 of human RhoB (NP_004031.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
ARH6; ARHB; RHOH6; MST081; MSTP081; RhoB
Positive Sample:
Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cleavage furrow, Late endosome membrane, Lipid-anchor, Nucleus.
Calculated MW:
22kDa
Observed MW:
22kDa
Predicted to enable GTP binding activity; GTPase activity; and protein kinase binding activity. Involved in several processes, including cellular response to hydrogen peroxide; cellular response to ionizing radiation; and regulation of cell migration. Located in cleavage furrow and endosome membrane. Biomarker of breast cancer.
Purification Method:
Affinity purification
Gene ID:
388
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain, using RhoB pAb (CAB16762) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
