The RhoA Polyclonal Antibody (CAB15641) is specifically designed for research involving RhoA, a small GTPase protein that plays a key role in regulating various cellular processes such as cell migration, cell adhesion, and cell shape. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot applications. It binds to the RhoA protein, enabling detection and analysis in a variety of cell types, making it an essential tool for studies in cell biology and cancer research.
RhoA is known for its involvement in cytoskeletal dynamics and cellular signaling pathways that are essential for normal cell function and development. Dysregulation of RhoA has been implicated in cancer progression, cardiovascular diseases, and neurological disorders, making it a promising target for therapeutic intervention. Understanding the role of RhoA in these diseases is crucial for the development of targeted therapies aimed at restoring normal cellular function and tissue homeostasis.
Product Name:
[KO Validated] RhoA Rabbit Polyclonal Antibody
SKU:
CAB15641
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RhoA (NP_001655.1).
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.
Purification Method:
Affinity purification
Gene ID:
387
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and RhoA knockout (KO) HeLa cells, using [KO Validated] RhoA Rabbit pAb (CAB15641) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
