The Rheb Polyclonal Antibody (CAB1165) is a valuable tool for researchers studying Rheb, a key protein involved in the regulation of cell growth and proliferation. This antibody, produced in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.Rheb, also known as Ras homolog enriched in brain, is a critical component of the mTOR signaling pathway, which plays a key role in controlling cell metabolism, growth, and survival. Dysregulation of this pathway has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes.
By specifically binding to Rheb protein, this antibody enables researchers to detect and analyze Rheb expression in different cell types, providing valuable insights into its function and potential therapeutic targets. Understanding the role of Rheb in cellular processes is essential for developing targeted therapies for diseases associated with mTOR pathway dysfunction.
Product Name:
RheB Rabbit Polyclonal Antibody
SKU:
CAB1165
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human RheB (NP_005605.1).
This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
Purification Method:
Affinity purification
Gene ID:
6009
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RheB antibody (CAB1165) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
