The RHBDD3 Polyclonal Antibody (PAC063403) is a valuable tool for researchers studying the RHBDD3 protein, an important molecule involved in cellular processes such as protein degradation and regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the RHBDD3 protein, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for studies in cell biology and molecular biology.The RHBDD3 protein, also known as Rhomboid domain-containing protein 3, plays a crucial role in intramembrane proteolysis, a process involved in the cleavage of membrane proteins.
Its involvement in protein degradation pathways makes it a key player in regulating important cellular functions. Research on RHBDD3 is essential for gaining insights into mechanisms underlying various diseases and conditions, including cancer, neurodegenerative disorders, and developmental abnormalities. By studying the function and regulation of the RHBDD3 protein, researchers can uncover potential therapeutic targets for intervention in these health-related contexts.
Western Blot. Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate. All lanes: RHBDD3 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41 kDa. Observed band size: 41 kDa.
Immunofluorescence staining of U251 cells with PACO63403 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO63403 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Synonyms:
Rhomboid domain-containing protein 3, RHBDD3, C22orf3
UniProt Protein Function:
RHBDD3: Protein type: Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 22q12.2Cellular Component: integral to membraneMolecular Function: serine-type endopeptidase activityBiological Process: response to xenobiotic stimulus; regulation of protein secretion; regulation of acute inflammatory response; negative regulation of natural killer cell activation; positive regulation of protein catabolic process; MAPKKK cascade; liver development; proteolysis
