The RGS14 Polyclonal Antibody (CAB9962) is a valuable tool for researchers studying the regulator of G protein signaling 14 (RGS14) protein. RGS14 is known for its role in modulating G protein-coupled receptor signaling pathways, making it a crucial regulator of various cellular processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting RGS14, researchers can detect and analyze this protein in a variety of cell types, providing valuable insights into signaling cascades and potential therapeutic targets.
The RGS14 Polyclonal Antibody is particularly useful in studies related to signal transduction, cell signaling, and drug discovery. Its ability to specifically bind to RGS14 enables detailed investigation into the mechanisms by which this protein influences cellular responses, offering opportunities for further understanding of disease pathways and potential interventions.
Product Name:
RGS14 Rabbit Polyclonal Antibody
SKU:
CAB9962
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 230-510 of human RGS14 (NP_006471.2).
This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Purification Method:
Affinity purification
Gene ID:
10636
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RGS14 Rabbit pAb (CAB9962) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
