The RGS12 Polyclonal Antibody (PAC022562) is a valuable tool for researchers studying RGS12, a regulator of G protein signaling involved in various cellular processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the RGS12 protein, this antibody allows for the detection and analysis of RGS12 expression in different cell types, making it an essential component for studies in cell signaling and signaling pathways.RGS12, also known as Regulator of G protein signaling 12, plays a crucial role in modulating G protein-coupled receptor signaling and downstream cellular responses.
Its involvement in multiple signaling cascades makes it a key player in processes such as cell proliferation, differentiation, and migration. Research into RGS12 function is particularly important for understanding its role in diseases such as cancer, cardiovascular disorders, and neurological conditions, where aberrant signaling pathways contribute to pathology. By elucidating the mechanisms by which RGS12 influences cellular signaling, this antibody paves the way for the development of targeted therapies that can manipulate these pathways for therapeutic benefit.
Antibody Name:
RGS12 Antibody (PACO22562)
Antibody SKU:
PACO22562
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from N-terminal of human RGS12.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from 3T3 cells, using RGS12 antibody.
Background:
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. soform 5:Behaves as a cell cycle-dependent transcriptional repressor, promoting inhibition of S-phase DNA synthesis.
Synonyms:
regulator of G-protein signaling 12;
UniProt Protein Function:
RGS12: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. 4 isoforms of the human protein are produced by alternative splicing.Protein type: GAPs, misc.; GAPsChromosomal Location of Human Ortholog: 4p16.3Cellular Component: nuclear matrix; condensed nuclear chromosome; cytoplasm; nucleolus; plasma membrane; nucleusMolecular Function: receptor signaling protein activity; GTPase regulator activity; GTPase activator activityBiological Process: transcription, DNA-dependent; regulation of catalytic activity; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the 'regulator of G protein signaling' (RGS) gene family. The encoded protein may function as a guanosine triphosphatase (GTPase)-activating protein as well as a transcriptional repressor. This protein may play a role in tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their biological nature has not been determined. [provided by RefSeq]
