The RFXANK Antibody (PAC057944) is a polyclonal antibody designed for research involving RFXANK, a transcription factor involved in regulating the expression of major histocompatibility complex class II genes. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.RFXANK plays a crucial role in the immune system by controlling the expression of genes involved in antigen presentation and immune response. Dysregulation of RFXANK has been linked to various diseases, including autoimmune disorders and immune deficiencies.
Understanding the role of RFXANK in the immune system is essential for developing targeted therapies for these conditions.This antibody binds specifically to RFXANK protein, enabling researchers to detect and analyze its expression in different cell types. It is an invaluable tool for studies in immunology and autoimmune research, providing insights into the mechanisms underlying immune dysregulation and potential therapeutic targets.
Western Blot. Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate. All lanes: RFXANK antibody at 6.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 25, 26 kDa. Observed band size: 29 kDa.
Immunofluorescence staining of PC-3 cells with PACO57944 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO57944 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. May also potentiate the activation of RAF1 (By similarity).
Synonyms:
DNA-binding protein RFXANK (Ankyrin repeat family A protein 1) (Regulatory factor X subunit B) (RFX-B) (Regulatory factor X-associated ankyrin-containing protein), RFXANK, ANKRA1 RFXB
UniProt Protein Function:
RFXANK: Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (MHC2TA). May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. Isoform RFX-B-delta5 is not involved in the positive regulation of MHC class II genes. Defects in RFXANK are a cause of bare lymphocyte syndrome type 2 (BLS2); also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-binding; Transcription, coactivator/corepressorChromosomal Location of Human Ortholog: 19p12Cellular Component: cytoplasm; intercellular bridge; nucleoplasm; nucleusMolecular Function: transcription cofactor activityBiological Process: regulation of transcription from RNA polymerase II promoterDisease: Bare Lymphocyte Syndrome, Type Ii
UniProt Protein Details:
NCBI Summary:
Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2013]
