RFXANK Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
RFXANK Antibody
SKU:
PACO57944
Size:
50μg
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant Human DNA-binding protein RFXANK protein (3-84AA)
Immunogen Species:
Homo sapiens (Human)
Uniprot No:
O14593
Form:
Liquid
Tested Applications:
ELISAWBIHCIF
Recommended Dilution:
Application
Recommended Dilution
WB
1:500-1:5000
IHC
1:200-1:500
IF
1:50-1:200
Synonyms:
ANKRA1 antibody, Ankyrin repeat containing regulatory factor X associated protein antibody, Ankyrin repeat family A protein 1 antibody, BLS antibody, DNA-binding protein RFXANK antibody, F14150_1 antibody, MGC138628 antibody, Regulatory factor X associated ankyrin containing protein antibody, Regulatory factor X subunit B antibody, Regulatory factor X, ankyrin repeat containing antibody, Regulatory factor X-associated ankyrin-containing protein antibody, RFX Bdelta4 antibody, RFX-B antibody, RFXANK antibody, RFXB antibody, RFXB delta 4 antibody, RFXK_HUMAN antibody
Western Blot Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate All lanes: RFXANK antibody at 6.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 25, 26 kDa Observed band size: 29 kDa
IHC image of PACO57944 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of PC-3 cells with PACO57944 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
