The RFX1 Polyclonal Antibody (CAB16066) is a valuable tool for researchers studying the function of the RFX1 transcription factor, a key regulator of gene expression. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By binding specifically to the RFX1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in fields such as molecular biology and developmental biology.
RFX1 plays a crucial role in the regulation of gene transcription, particularly in the context of immune response and cellular differentiation. Dysregulation of RFX1 has been implicated in various diseases, including cancer and autoimmune disorders, highlighting its potential as a therapeutic target. By gaining insight into the mechanisms by which RFX1 influences gene expression, researchers can further our understanding of disease pathogenesis and potentially identify novel treatment strategies.
Product Name:
RFX1 Rabbit Polyclonal Antibody
SKU:
CAB16066
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human RFX1 (NP_002909.4).
This gene encodes a member of the regulatory factor X (RFX) family of transcription factors, which are characterized by a winged-helix DNA-binding domain. The encoded transcription factor contains an N-terminal activation domain and a C-terminal repression domain, and may activate or repress target gene expression depending on cellular context. This transcription factor has been shown to regulate a wide variety of genes involved in immunity and cancer, including the MHC class II genes and genes that may be involved in cancer progression. This gene exhibits altered expression in glioblastoma and the autoimmune disease systemic lupus erythematosis (SLE).
Purification Method:
Affinity purification
Gene ID:
5989
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from LO2 cells, using RFX1 Rabbit pAb (CAB16066) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
