The RFWD2 Polyclonal Antibody (CAB10463) is a valuable tool for researchers studying RFWD2, a protein involved in DNA damage response and genomic stability. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to RFWD2, this antibody enables precise detection and analysis of the protein, making it an essential component of studies in genetics, cell biology, and cancer research.RFWD2, also known as E3 ubiquitin-protein ligase, plays a crucial role in maintaining genome integrity by regulating DNA repair pathways and cell cycle progression.
Dysregulation of RFWD2 has been linked to various genetic disorders and cancer development, highlighting its importance in cellular function and disease prevention. By studying the functions and interactions of RFWD2, researchers can gain insights into mechanisms of DNA repair and potential therapeutic targets for cancer treatment.
Product Name:
RFWD2 Rabbit Polyclonal Antibody
SKU:
CAB10463
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 582-731 of human RFWD2 (NP_071902.2).
Enables ubiquitin protein ligase activity. Involved in positive regulation of proteasomal ubiquitin-dependent protein catabolic process; proteasome-mediated ubiquitin-dependent protein catabolic process; and response to ionizing radiation. Part of Cul4A-RING E3 ubiquitin ligase complex.
Purification Method:
Affinity purification
Gene ID:
64326
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RFWD2 antibody (CAB10463) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
