The RFNG Polyclonal Antibody (PAC062839) is a valuable tool for researchers studying the RFNG protein, a key regulator in the Notch signaling pathway. This antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.RFNG is involved in the glycosylation of Notch receptors, playing a crucial role in modulating Notch signaling activity. Dysregulation of this pathway has been linked to various diseases, including cancer and developmental disorders.
By targeting RFNG with this antibody, researchers can gain insights into the mechanisms underlying Notch signaling and its impact on cellular processes.With its high specificity and sensitivity, the RFNG Polyclonal Antibody is an essential tool for investigations into the Notch signaling pathway and its implications in disease pathology. Its versatility in detecting RFNG expression in different cell types makes it an ideal choice for studies in developmental biology, oncology, and other fields exploring Notch signaling.
Western Blot. Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, PC3 whole cell lysate, Rat brain tissue. All lanes: RFNG antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37 kDa. Observed band size: 37 kDa.
IHC image of PACO62839 diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. May be involved in limb formation and in neurogenesis (By similarity).
RFNG: Glycosyltransferase that initiates the elongation of O- linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. May be involved in limb formation and in neurogenesis. Belongs to the glycosyltransferase 31 family.Protein type: Motility/polarity/chemotaxis; EC 2.4.1.222; Transferase; Membrane protein, integralChromosomal Location of Human Ortholog: 17q25
