The RFFL Polyclonal Antibody (CAB6489) is a powerful tool for researchers studying the RFFL protein, an E3 ubiquitin-protein ligase involved in regulating cellular processes such as apoptosis and inflammation. Raised in rabbits, this antibody demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the RFFL protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component in immunology and cancer research studies.The RFFL protein plays a crucial role in cellular signaling pathways that control cell survival and death, making it a key target for investigation in diseases such as cancer and inflammatory disorders.
By understanding the function of RFFL, researchers can uncover new insights into the mechanisms that drive these diseases and potentially identify new therapeutic targets for intervention. The RFFL Polyclonal Antibody is an invaluable tool in this research, providing researchers with the ability to study RFFL expression and activity in a precise and reliable manner.
Product Name:
RFFL Rabbit Polyclonal Antibody
SKU:
CAB6489
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RFFL (NP_001017368.1).
Enables enzyme binding activity; p53 binding activity; and ubiquitin protein ligase activity. Involved in cellular protein metabolic process; negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis; and negative regulation of signal transduction. Located in endosome membrane and plasma membrane.
Purification Method:
Affinity purification
Gene ID:
117584
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of U-251MG cells, using RFFL antibody (CAB6489) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 40s.
