The RFC4 Polyclonal Antibody (CAB5485) is a valuable tool for researchers studying RFC4, a protein involved in DNA replication and repair processes. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. It specifically targets the RFC4 protein, allowing for accurate detection and analysis in a variety of cell types.RFC4 plays a crucial role in maintaining genomic stability by assisting in the replication of DNA and ensuring proper repair of damaged DNA.
Dysregulation of RFC4 expression has been linked to various diseases, including cancer and genetic disorders. By studying the function of RFC4, researchers can gain insights into these diseases and potentially develop targeted therapies.Overall, the RFC4 Polyclonal Antibody is an essential tool for investigating the role of RFC4 in DNA replication and repair pathways, making it ideal for research in the fields of genetics, molecular biology, and cancer biology.
Product Name:
RFC4 Rabbit Polyclonal Antibody
SKU:
CAB5485
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
A1; RFC37; RFC4
Positive Sample:
HeLa,SW480,22Rv1,SW620,K-562,HepG2,MCF7
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
40kDa
Observed MW:
40kDa
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Purification Method:
Affinity purification
Gene ID:
5984
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RFC4 Rabbit pAb (CAB5485) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
