The RFC3 Polyclonal Antibody (CAB4075) is a valuable tool for researchers studying RFC3, a protein involved in DNA replication and repair processes. This polyclonal antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.RFC3, part of the Replication Factor C complex, plays a crucial role in loading the PCNA protein onto DNA during replication and repair processes. Dysregulation of RFC3 has been linked to genomic instability and cancer development. The RFC3 Polyclonal Antibody binds specifically to RFC3, enabling researchers to detect and analyze its expression in various cell types.
This makes it an ideal reagent for studies in molecular biology, cancer research, and DNA replication mechanisms.Understanding the function and regulation of RFC3 is essential for uncovering its role in disease development and for identifying potential therapeutic targets. By using the RFC3 Polyclonal Antibody, researchers can gain valuable insights into the molecular mechanisms underlying DNA replication and repair, paving the way for new advancements in cancer treatment and genomic stability research.
Product Name:
RFC3 Rabbit Polyclonal Antibody
SKU:
CAB4075
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RFC3 (NP_002906.1).
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
5983
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RFC3 antibody (CAB4075) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
