The RFC1 Polyclonal Antibody (CAB1625) is a powerful tool for researchers studying RFC1, a protein known for its role in DNA replication and repair. This antibody, produced in rabbits, is highly specific to human samples and has been validated for Western blot applications. By binding to the RFC1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential tool for research in fields such as molecular biology and genetics.RFC1, also known as Replication Factor C Subunit 1, is a key player in the multi-protein complex involved in DNA replication and repair processes. Understanding the function and regulation of RFC1 is crucial for unraveling the mechanisms underlying cell division and genome stability.
Research on RFC1 can provide insights into diseases such as cancer, where aberrant DNA replication can lead to genomic instability and tumor formation.Overall, the RFC1 Polyclonal Antibody (CAB1625) offers researchers a reliable and specific tool for studying RFC1 and its functions in various cellular processes. Its application in Western blot assays allows for accurate detection and quantification of RFC1 levels, furthering our understanding of this important protein in DNA maintenance and cell proliferation.
Product Name:
RFC1 Rabbit Polyclonal Antibody
SKU:
CAB1625
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-700 of human RFC1 (NP_001191676.1).
This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Purification Method:
Affinity purification
Gene ID:
5981
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RFC1 antibody (CAB1625) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
