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Anti-Ret Antibody (CAB0018)

SKU:
CAB0018
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
  • Anti-Ret Antibody (CAB0018)
  • Immunofluorescence analysis of Neuro-2a cells using Ret Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-SY5Y cells using Ret Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat brain, using Ret antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
€139 - €329
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Description

Antibody Name:Anti-Ret Antibody (CAB0018)
Antibody SKU:CAB0018
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human Ret (NP_066124.1).
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human Ret (NP_066124.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DISK DLEK MMVK RRDY LDLA ASTP SDSL IYDD GLSE EETP LVDC NNAP LPRA LPST WIEN KLYG MSDP NWPG ESPV PLTR ADGT NTGF PRYP NDSV YANW M
Cellular Location:Cell membrane, Endosome membrane, Single-pass type I membrane protein
Calculated MW:119kDa/124kDa
Observed MW:150KDa
Synonyms:RET, CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1, RET51
Background:This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed.
Western blotWestern blot
Immunofluorescence analysis of Neuro-2a cells using Ret Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of Neuro-2a cells using Ret Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of SH-SY5Y cells using Ret Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of SH-SY5Y cells using Ret Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of Rat brain, using Ret antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Western blot analysis of extracts of Rat brain, using Ret antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

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