The REST Polyclonal Antibody (CAB7161) is specifically designed for research involving the RE1-silencing transcription factor (REST), a key regulator of gene expression in the nervous system. This antibody, raised in rabbits, is highly reactive with human and mouse samples and has been validated for use in various applications including Western blot, immunofluorescence, and immunohistochemistry.REST is known for its role in repressing neuronal genes in non-neuronal cells, making it a crucial factor in neural development and function.
Dysregulation of REST has been implicated in various neurological disorders such as Alzheimer's disease, epilepsy, and Huntington's disease. By targeting REST with this antibody, researchers can study its expression and localization in different cell types, shedding light on its function and potential therapeutic implications in neurodegenerative diseases.
Product Name:
REST/NRSF Rabbit Polyclonal Antibody
SKU:
CAB7161
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-130 of human REST/NRSF (NP_005603.3).
This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
5978
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using REST/NRSF Rabbit pAb (CAB7161) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
