The REST (NRSF) Rabbit Monoclonal Antibody (CAB2415) is a high-quality antibody designed for use in research involving the RE1-silencing transcription factor (REST), also known as neuron-restrictive silencer factor (NRSF). This protein plays a critical role in gene regulation, specifically in neural development and function.Raised in rabbits, this monoclonal antibody is highly specific and reactive with human samples, making it a valuable tool for studies in neuroscience, neurology, and related fields. It has been validated for use in Western blot applications, allowing for the detection and analysis of REST/NRSF protein levels in various cell types and tissues.
The REST/NRSF antibody allows researchers to investigate the role of this transcription factor in gene silencing and neural processes, providing valuable insights into its function and potential implications in neurological disorders, such as Alzheimer's disease, Parkinson's disease, and epilepsy. Understanding the mechanisms of action of REST/NRSF is crucial for developing targeted therapies and interventions for these conditions.Overall, the REST (NRSF) Rabbit Monoclonal Antibody (CAB2415) is a reliable tool for studying the intricacies of gene regulation in the nervous system and exploring new avenues for therapeutic advancements in neuroscience research.
Product Name:
REST/NRSF Rabbit Monoclonal Antibody
SKU:
CAB2415
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human REST/NRSF (Q13127).
Sequence:
KGEP HGLE NMEL RSLE LSVV EPQP VFEA SGAP DIYS SNKD LPPE TPGA EDKG KSSK TKPF RCKP CQYE AESE EQFV HHIR VHSA KKFF VEES AEKQ AKAR E
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
WT6; XBR; HGF5; NRSF; DFNA27; GINGF5; REST/NRSF
Positive Sample:
HeLa,U-87MG,Mouse brain,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Nucleus
Calculated MW:
122kDa
Observed MW:
170kDa
This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
5978
Clone Number:
ARC0755
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using REST/NRSF Rabbit mAb (CAB2415) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
