The Rent1/Hupf1 Rabbit Monoclonal Antibody (CAB5071) is a highly specific and sensitive tool for studying the Rent1/Hupf1 protein in various biological samples. Rent1/Hupf1 is a key regulator of mRNA stability and has been linked to important cellular processes such as RNA degradation and gene expression.This monoclonal antibody, derived from rabbit hosts, has been extensively validated for use in Western blot applications, enabling researchers to reliably detect and analyze Rent1/Hupf1 protein levels in different cell types. Its high reactivity with human samples makes it an ideal choice for studies in molecular biology, RNA biology, and gene regulation.
The Rent1/Hupf1 Rabbit Monoclonal Antibody (CAB5071) offers researchers a valuable tool for investigating the role of Rent1/Hupf1 in various biological processes, including RNA surveillance mechanisms, mRNA decay pathways, and post-transcriptional gene regulation. Its specificity and sensitivity make it a reliable choice for researchers aiming to unravel the complex functions of Rent1/Hupf1 in health and disease.
Product Name:
UPF1/RENT1 Rabbit Monoclonal Antibody
SKU:
CAB5071
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UPF1/RENT1 (Q92900).
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5976
Clone Number:
ARC1268
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using UPF1/RENT1 Rabbit mAb (CAB5071) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
