The REN1 Polyclonal Antibody (PAC06491) is a valuable tool for researchers studying the REN1 protein, which plays a key role in the renin-angiotensin system involved in blood pressure regulation and electrolyte balance. This antibody is produced in rabbits and is highly specific to human samples, making it ideal for use in Western blot applications. By binding to the REN1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it a crucial asset for studies in cardiovascular disease, hypertension, and kidney function.
REN1, also known as renin, is a protease enzyme that catalyzes the conversion of angiotensinogen to angiotensin I, a crucial step in the regulation of blood pressure and fluid balance. Dysregulation of the renin-angiotensin system is linked to various cardiovascular disorders, making REN1 a promising target for therapeutic interventions. Researchers investigating the role of REN1 in disease pathology and treatment development will find this antibody indispensable for their studies.
Western Blot. Positive WB detected in: Rat Heart tissue, Rat Liver tissue. All lanes: Ren1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa.
IHC image of PACO64491 diluted at 1:100 and staining in paraffin-embedded rat kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Background:
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:EC 3.4.23.15; Secreted, signal peptide; Secreted; Protease
