Anti-NF-kB p65 Antibody (CAB2547)
- SKU:
- CAB2547
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
Anti-NF-kB p65 Antibody (CAB2547)
The RelA Polyclonal Antibody (CAB2547) is a valuable tool for researchers studying the NF-kB transcription factor RelA, which plays a key role in regulating immune and inflammatory responses. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the RelA protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in immunology, inflammation, and cancer research.RelA, also known as p65, is a crucial component of the NF-kB signaling pathway, which is involved in the regulation of genes related to immunity, inflammation, and cell survival.
Dysregulation of NF-kB signaling, including aberrant activation of RelA, has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory conditions. By understanding the role of RelA in these processes, researchers can uncover new insights into disease mechanisms and potential therapeutic targets.Overall, the RelA Polyclonal Antibody (CAB2547) provides researchers with a reliable tool for studying the function of the NF-kB transcription factor RelA in various physiological and pathological contexts. Its high specificity and sensitivity make it a valuable asset for investigations into the intricate mechanisms of immune regulation and inflammatory pathways.
Antibody Name: | Anti-NF-kB p65 Antibody |
Antibody SKU: | CAB2547 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB IHC IF IP |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-180 of human NF-kB p65 (NP_068810.3). |
Application: | WB IHC IF IP |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HeLa, 293T, MCF7, Mouse lung, Mouse kidney, Rat lung, Rat kidney |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-180 of human NF-kB p65 (NP_068810.3). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | RSTD TTKT HPTI KING YTGP GTVR ISLV TKDP PHRP HPHE LVGK DCRD GFYE AELC PDRC IHSF QNLG IQCV KKRD LEQA ISQR IQTN NNPF QVPI EEQR GDYD LNAV RLCF QVTV RDPS GRPL RLPP VLS |
Gene ID: | 5970 |
Uniprot: | Q04206 |
Cellular Location: | Cytoplasm, Nucleus |
Calculated MW: | 58kDa/59kDa/60kDa |
Observed MW: | 65KDa |
Synonyms: | NFKB3, p65, NF-kB p65, RELA, CMCU |
Background: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. |
UniProt Protein Function: | NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified. |
UniProt Protein Details: | Protein type:DNA-binding; Nuclear receptor co-regulator; Transcription factor Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex Molecular Function:actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inflammatory response; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to organic substance; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway |
NCBI Summary: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
UniProt Code: | Q04206 |
NCBI GenInfo Identifier: | 62906901 |
NCBI Gene ID: | 5970 |
NCBI Accession: | Q04206.2 |
UniProt Secondary Accession: | Q04206,Q6GTV1, Q6SLK1, |
UniProt Related Accession: | Q04206 |
Molecular Weight: | 59,910 Da |
NCBI Full Name: | Transcription factor p65 |
NCBI Synonym Full Names: | RELA proto-oncogene, NF-kB subunit |
NCBI Official Symbol: | RELAÂ Â |
NCBI Official Synonym Symbols: | p65; NFKB3Â Â |
NCBI Protein Information: | transcription factor p65 |
UniProt Protein Name: | Transcription factor p65 |
UniProt Synonym Protein Names: | Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
Protein Family: | Proline-rich P65 protein |
UniProt Gene Name: | RELAÂ Â |