The RELA Polyclonal Antibody (CAB22684) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications, allowing for the detection and analysis of RELA in various cell types.RELA, also known as NF-kappa-B p65 subunit, plays a crucial role in regulating the immune response and inflammatory processes. Its involvement in these pathways makes it a key molecule to study in the context of diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
RELA Polyclonal Antibody
SKU:
CAB22684
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Enables several functions, including DNA-binding transcription factor activity, RNA polymerase II-specific; actinin binding activity; and ankyrin repeat binding activity. Involved in several processes, including negative regulation of protein sumoylation; postsynapse to nucleus signaling pathway; and regulation of transcription, DNA-templated. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; cellular response to lipopolysaccharide; and positive regulation of interleukin-12 production. Located in several cellular components, including chromatin; cytosol; and glutamatergic synapse. Is expressed in several structures, including alimentary system; early conceptus; genitourinary system; musculature; and skin. Human ortholog(s) of this gene implicated in ductal carcinoma in situ; lung non-small cell carcinoma; lymphoma; and renal cell carcinoma. Orthologous to human RELA (RELA proto-oncogene, NF-kB subunit).
Purification Method
Affinity purification
Gene ID
19697
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type(WT) and RELA knockout (KO) HeLa cells, using [KO Validated] RELA Rabbit pAb (CAB22684) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
