The RELA Polyclonal Antibody (CAB22676) offered by Assay Genie is a powerful tool for researchers studying the NF-κB protein RELA, a key regulator of immune and inflammatory responses. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications. By targeting the RELA protein, this antibody enables the detection and analysis of RELA in various cell types, facilitating research in immunology, inflammation, and cancer biology.RELA is a pivotal component of the NF-κB signaling pathway, which is instrumental in regulating the expression of genes involved in immune responses, cell survival, and inflammation.
Dysregulation of the NF-κB pathway and aberrant RELA activity have been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory conditions. By studying RELA expression and function using this antibody, researchers can gain valuable insights into the molecular mechanisms underlying these diseases and identify potential therapeutic targets.
Enables several functions, including DNA-binding transcription factor activity, RNA polymerase II-specific; actinin binding activity; and ankyrin repeat binding activity. Involved in several processes, including negative regulation of protein sumoylation; postsynapse to nucleus signaling pathway; and regulation of transcription, DNA-templated. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; cellular response to lipopolysaccharide; and positive regulation of interleukin-12 production. Located in several cellular components, including chromatin; cytosol; and glutamatergic synapse. Is expressed in several structures, including alimentary system; early conceptus; genitourinary system; musculature; and skin. Human ortholog(s) of this gene implicated in ductal carcinoma in situ; lung non-small cell carcinoma; lymphoma; and renal cell carcinoma. Orthologous to human RELA (RELA proto-oncogene, NF-kB subunit).
Purification Method:
Affinity purification
Gene ID:
19697
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using RELA Rabbit pAb (CAB22676) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
