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Anti-NF-kB p65 Antibody (CAB16271)

SKU:
CAB16271
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 2 Anti-NF-kB p65 Antibody CAB16271
  • Immunology Antibodies 2 Anti-NF-kB p65 Antibody CAB16271
€139 - €419
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Description

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Anti-NF-kB p65 Antibody (CAB16271)

The RELA Polyclonal Antibody (CAB16271) is a powerful tool for researchers studying the RELA protein, a key player in the NF-kB signaling pathway. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications. By targeting the RELA protein, this antibody enables precise detection and quantification in a variety of cell types, providing valuable insights into cellular signaling pathways and their role in immune response and inflammation. RELA, also known as p65, is a crucial transcription factor involved in the regulation of genes related to immunity, inflammation, and cell survival.

Dysregulation of RELA activity has been linked to various diseases, including cancer, autoimmune disorders, and chronic inflammatory conditions. By studying the function of RELA using this antibody, researchers can uncover new therapeutic targets and strategies for treating these complex diseases. Overall, the RELA Polyclonal Antibody (CAB16271) is an essential tool for researchers interested in understanding the role of the NF-kB signaling pathway and its impact on immune response and disease development.

Antibody Name:Anti-NF-kB p65 Antibody
Antibody SKU:CAB16271
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human NF-kB p65
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, A-549, MCF7, Mouse lung, Mouse spleen, Rat lung
Immunogen:A synthetic peptide of human NF-kB p65
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5970
Uniprot:Q04206
Cellular Location:Cytoplasm, Nucleus
Calculated MW:58kDa/59kDa/60kDa
Observed MW:65KDa
Synonyms:NFKB3, p65, NF-kB p65, RELA, CMCU
Background:NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.
UniProt Protein Details:

Protein type:DNA-binding; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function:actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inflammatory response; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to organic substance; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway

NCBI Summary:NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:Q04206
NCBI GenInfo Identifier:62906901
NCBI Gene ID:5970
NCBI Accession:Q04206.2
UniProt Secondary Accession:Q04206,Q6GTV1, Q6SLK1,
UniProt Related Accession:Q04206
Molecular Weight:59,910 Da
NCBI Full Name:Transcription factor p65
NCBI Synonym Full Names:RELA proto-oncogene, NF-kB subunit
NCBI Official Symbol:RELA  
NCBI Official Synonym Symbols:p65; NFKB3  
NCBI Protein Information:transcription factor p65
UniProt Protein Name:Transcription factor p65
UniProt Synonym Protein Names:Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Protein Family:Proline-rich P65 protein
UniProt Gene Name:RELA  
Anti-NF-kB p65 Antibody (CAB16271)Western blot analysis of extracts of various cell lines, using NF-kB p65 Rabbit pAb (CAB16271) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-NF-kB p65 Antibody (CAB16271)Western blot analysis of extracts of various cell lines, using NF-kB p65 Rabbit pAb (CAB16271) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

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