The RELA Polyclonal Antibody (CAB11201) is specifically designed for researching RELA, a transcription factor involved in the regulation of genes related to immune response and inflammation. Produced in rabbits, this antibody demonstrates high reactivity with human samples and has been validated for use in Western blot applications. RELA is a key player in the NF-κB pathway, a signaling cascade critical for the expression of genes involved in immunity, inflammation, and cell survival. By binding to RELA, this antibody allows for precise detection and analysis of the protein in various cell types, making it a valuable tool for researchers in the fields of immunology and cancer biology.
The role of RELA in immune regulation and inflammatory responses makes it a promising target for studying diseases such as cancer, autoimmune disorders, and chronic inflammation. Understanding the function and regulation of RELA is essential for developing therapeutic strategies that modulate immune responses and combat disease progression.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5970
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NF-kB p65/RelA Rabbit pAb (CAB11201) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
