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Rel (Phospho-Ser503) Colorimetric Cell-Based ELISA Kit (CBCAB00484)

SKU:
CBCAB00484
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human
Detection Method:
Colorimetric
$1,152

Description

system_update_altDatasheetMSDS

Rel (Phospho-Ser503)Colorimetric Cell-Based ELISA Kit

The REL Phospho-Ser503 Colorimetric Cell-Based ELISA Kit is specifically designed for the detection of phosphorylated serine 503 levels in cell lysates and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for research applications.Phosphorylation of serine 503 is known to be involved in regulating cellular signaling pathways and can impact various cellular processes. By studying the phosphorylation status of serine 503, researchers can gain valuable insights into the mechanisms underlying cell signaling and potential therapeutic targets for diseases such as cancer, inflammatory disorders, and neurodegenerative diseases.

Overall, the REL Phospho-Ser503 Colorimetric Cell-Based ELISA Kit is an essential tool for researchers studying cell signaling pathways and investigating the role of phosphorylated serine 503 in various disease states. It offers reliable and precise measurement of phosphorylated serine 503 levels, providing valuable data for advancing scientific research and drug development efforts.

Product Name:Rel (Phospho-Ser503) Colorimetric Cell-Based ELISA
Product Code:CBCAB00484
ELISA Type:Cell-Based
Target:Rel (Phospho-Ser503)
Reactivity:Human
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The Rel (Phospho-Ser503) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Rel protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Rel in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Rel phosphorylation.

Qualitative determination of Rel (Phospho-Ser503) concentration is achieved by an indirect ELISA format. In essence, Rel (Phospho-Ser503) is captured by Rel (Phospho-Ser503)-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 5966, UniProt ID: Q04864, OMIM: 164910, Unigene: Hs.631886
Gene Symbol:REL
Sub Type:Phospho
UniProt Protein Function:REL: a proto-oncogenic transcription factor of the nuclear factor-kappaB (NFkB) group. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. May play a role in differentiation and lymphopoiesis.
UniProt Protein Details:

Protein type:Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 2p13-p12

Cellular Component: cytosol; nucleoplasm; nucleus; transcription factor complex

Molecular Function:chromatin binding; protein binding; transcription factor activity

Biological Process: cytokine production; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; negative regulation of interferon-beta production; negative regulation of transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; response to cytokine stimulus; transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a protein that belongs to the Rel homology domain/immunoglobulin-like fold, plexin, transcription factor (RHD/IPT) family. Members of this family regulate genes involved in apoptosis, inflammation, the immune response, and oncogenic processes. This proto-oncogene plays a role in the survival and proliferation of B lymphocytes. Mutation or amplification of this gene is associated with B-cell lymphomas, including Hodgkin's lymphoma. Single nucleotide polymorphisms in this gene are associated with susceptibility to ulcerative colitis and rheumatoid arthritis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]
UniProt Code:Q04864
NCBI GenInfo Identifier:548720
NCBI Gene ID:5966
NCBI Accession:Q04864.1
UniProt Secondary Accession:Q04864,Q17RU2, Q2PNZ7, Q6LDY0,
UniProt Related Accession:Q04864
Molecular Weight:65,225 Da
NCBI Full Name:Proto-oncogene c-Rel
NCBI Synonym Full Names:v-rel avian reticuloendotheliosis viral oncogene homolog
NCBI Official Symbol:REL  
NCBI Official Synonym Symbols:C-Rel  
NCBI Protein Information:proto-oncogene c-Rel
UniProt Protein Name:Proto-oncogene c-Rel
Protein Family:Nuclear factor
UniProt Gene Name:REL  
UniProt Entry Name:REL_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-Rel (Phospho-Ser503) Antibody, Anti-Rel Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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