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Reduced Glutathione (GSH) Colorimetric Assay Kit (MAES0042)

SKU:
MAES0042
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Tissue, Cells
Sensitivity:
2 umol/L
Range:
2-100 umol/L
Assay Time:
25 min
Shipping:
Gel Pack
Research Area:
Oxidative Stress
Antioxidant
Diabetes & Obesity
€289

Description

system_update_altDatasheet
Product Name:Reduced Glutathione (GSH) Assay Kit - Colorimetric
Product Code:MAES0042
Product Size:96 Assays
Sample Type:Serum, plasma, cell culture supernatant, tissue, cells
Assay Time:25 min
Instrument:Microplate reader
Sensitivity:2 µmol/L
Detection Range:2-100 µmol/L
Recovery Rate:96
Inter CV:3.2
Inter CV:1.9

Reduced glutathione (GSH) is a kind of low molecular scavenger, which can remove O2-, H2O2, LOOH. GSH is a small molecule peptide which composed of glutamic acid, glycine and cysteine, and it is the main thiol compound of non-protein in the organization. GSH is the substrate of GSH-Px and GSH-ST which is indispensable for decomposing hydrogen peroxide of these two enzymes, and it can stabilize the enzyme containing thiol and prevent hemoglobin and other auxiliary factors from the oxidative damage. Recently, it is proved that GSH is also involved in the recovery of VE to the reduction state. When lacking or depletion of GSH, it may cause producing toxic effects or increasing the toxic effects of many chemicals or environmental factors. It may be related to the increase of oxidative damage, so the amount of GSH is a vital factor to measure the body's antioxidant ability. GSH plays an important role in the researches process of prevention, recovery and treatment of atherosclerosis, coronary heart disease, anti-aging, anti-tumor, prevention, prevention of Alzheimer's disease and other diseases.

Reduced glutathione (GSH) can react with dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.

Shelf Life:6 Months
Storage:2-8°C
Shipping:Gel Pack
Research Area:Oxidative stress

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