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Recoverin Colorimetric Cell-Based ELISA

SKU:
CBCAB01003
Product Type:
ELISA Kit
ELISA Type:
Cell Based
Research Area:
Signal Transduction
Reactivity:
Human
Mouse
Detection Method:
Colorimetric
€599
Frequently bought together:

Description

system_update_altDatasheet

Recoverin Colorimetric Cell-Based ELISA

The Recoverin Colorimetric Cell-Based ELISA Kit is a cutting-edge tool for the precise measurement of recoverin levels in cell lysates and culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and dependable results, making it perfect for a variety of research purposes.Recoverin is a key protein involved in the visual process, particularly in the regulation of phototransduction in the retina. Its dysfunction has been linked to various retinal disorders, making it a valuable biomarker for studying these conditions and developing new treatments.

This innovative kit offers researchers a powerful tool for exploring the role of recoverin in vision and eye diseases, providing valuable insights that could lead to advancements in diagnosis and therapy. Order your Recoverin Colorimetric Cell-Based ELISA Kit today and take your research to the next level.

Product Name:Recoverin Colorimetric Cell-Based ELISA
Product Code:CBCAB01003
ELISA Type:Cell-Based
Target:Recoverin
Reactivity:Human, Mouse
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nmStorage/Stability:4°C/6 Months
Format:96-Well Microplate

The Recoverin Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Recoverin protein expression profile in cells. The kit can be used for measuring the relative amounts of Recoverin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on Recoverin.

Qualitative determination of Recoverin concentration is achieved by an indirect ELISA format. In essence, Recoverin is captured by Recoverin-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 5957, UniProt ID: P35243, OMIM: 179618, Unigene: Hs.80539
Gene Symbol:RCVRN
Sub Type:None
UniProt Protein Function:RCV1: Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse. Belongs to the recoverin family.
UniProt Protein Details:

Protein type:Calcium-binding

Chromosomal Location of Human Ortholog: 17p13.1

Molecular Function:calcium ion binding; calcium sensitive guanylate cyclase activator activity

Biological Process: phototransduction, visible light; positive regulation of guanylate cyclase activity; regulation of calcium ion transport; regulation of rhodopsin mediated signaling; rhodopsin mediated signaling; signal transduction; visual perception

NCBI Summary:This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy. [provided by RefSeq, Jul 2008]
UniProt Code:P35243
NCBI GenInfo Identifier:464600
NCBI Gene ID:5957
NCBI Accession:P35243.2
UniProt Secondary Accession:P35243,Q53XL0,
UniProt Related Accession:P35243
Molecular Weight:23,130 Da
NCBI Full Name:Recoverin
NCBI Synonym Full Names:recoverin
NCBI Official Symbol:RCVRN  
NCBI Official Synonym Symbols:RCV1  
NCBI Protein Information:recoverin
UniProt Protein Name:Recoverin
UniProt Synonym Protein Names:Cancer-associated retinopathy protein; Protein CAR
Protein Family:Recoverin
UniProt Gene Name:RCVRN  
UniProt Entry Name:RECO_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies (Anti-Recoverin Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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