SARS-CoV-2 Nucleocapsid Recombinant Protein (RPES0039)
- SKU:
- RPES0039
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Immunology
Description
Background
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
| Key Information | Description |
| Product Name: | SARS-Cov-2 Nucleocapsid Protein (His Tag) |
| Size: | 100µg, 1mg |
| SKU: | RPES0039 |
| Activity: | |
| Synonym: | 2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein |
| Protein Construction: | A DNA sequence encoding the 2019-nCoV Nucleocapsid Protein (YP_009724397.2(335Gly/Ala)) (Met1-Ala419) was expressed with a polyhistidine tag at the C-terminus. |
| Sequence: | Met1-Ala419 |
| Fusion tag: | C-6His |
| Accession: | YP_009724397.2 |
| Species: | virus |
| Expressed Host: | Baculovirus-Insect Cells |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity: | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin: | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20? for 3 months. |
| Mol Mass: | 47.08 kDa |
| AP Mol Mass: | |
| Formulation: | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10%glycerol. |
| Reconstitution: | Please refer to the printed manual for detailed information. |
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