The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.
Product Name:
Rat TNFR1/TNFRSF1A Recombinant Protein (RPES0729)
Product Code:
RPES0729
Size:
50µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
TNFRSF1A,Tnfr-1,Tnfr1
Accession:
P22934
Sequence:
Met1-Ala211
Fusion tag:
C-Fc
Activity:
1. Measured by its ability to inhibit TNF?-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of ratTNF?.2. Immobilized mouse TNFa (80-235) at 10 µg/ml (100 ?l/well) can bind rat TNFRSF1A-Fc, The EC50 of rat TNFRSF1A-Fc is 0.08-0.20 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat TNFRSF1A (P22934) (Met1-Ala211) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
47.9 kDa
AP Mol Mass:
59 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
