Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein which has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.
Product Name:
Rat SerpinF1/PEDF Recombinant Protein (RPES4113)
Product Code:
RPES4113
Size:
20µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
Dmrs91
Accession:
NP_808788.1
Sequence:
Met1-Thr418
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the rat Serpinf1 (NP_808788.1) (Met1-Thr418) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
45.9 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
