Recombinant Rat IL-10 Protein (Trx Tag) (RPES8230)
- SKU:
- RPES8230
- Size:
- 20μg
- Tag:
- N-Trx
- Reactivity:
- Rat
- Expression Host:
- E.coli
- Accession:
- P29456
Description
| Product Name: | Recombinant Rat IL-10 Protein (Trx Tag) |
| SKU: | RPES8230 |
| Size: | 20μg |
| Tag: | N-Trx |
| Reactivity: | Rat |
| Expression Host: | E.coli |
| Accession: | P29456 |
| Sequence: | Ser19-Asn178 |
| Calculated Molecular Weight: | 37.5 kDa |
| Observed Molecular Weight: | 37.5 kDa |
| Synonyms: | Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, |
| Endotoxin: | < 10 EU/mg of the protein as determined by the LAL method |
| Purity: | > 90% as determined by reducing SDS-PAGE. |
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.
| Bio-activity: | Not validated for activity |
| Formulation: | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution: | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Storage: | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
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