Ephrin type-A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines; however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas.
Product Name:
Rat EphA7/EHK3 Recombinant Protein (RPES0947)
Product Code:
RPES0947
Size:
50µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
EPHA7,Ehk-3,Ehk3
Accession:
NP_599158.1
Sequence:
Met1-Ser539
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA.Immobilized mouse EFNA4-His at 10 µg/ml (100 ?l/well) can bind rat EPHA7-Fc3, The EC50 of rat EPHA7-Fc3 is 10-30 ng/mL.
Endotoxin:
<1.0 EU per µg protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the rat Epha7 (NP_599158.1) (Met1-Ser539) was expressed with the Fc region of human IgG1 at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
58.9 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
