The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
Product Name:
Rat CD16a/FCGR3A Recombinant Protein (RPES1500)
Product Code:
RPES1500
Size:
50µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
Fcgr4
Accession:
ABO70343.1
Sequence:
Met 1-Pro 200
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized rat FCGR3A-His at 10 µg/ml (100 ?l/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 59.0-139.0 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat FCGR3A (ABO70343.1) extracellular domain (Met 1-Pro 200) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
21.7 kDa
AP Mol Mass:
28-33 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
