Cadherins are integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Type I cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of five subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherin 8, also known as CDH 8, is a type I I classical cadherin belonging to the cadherin superfamily. As mainly expressed in brain, CDH8 is found in certain nerve cell lines, such as retinoblasts, glioma cells and neuroblasts, and is putatively involved in synaptic adhesion, axon outgrowth and guidance. Human Cadherin 8 is a 799 amino acid single-pass type I transmembrane protein with a putative 29 aa signal sequence, and a 32 aa propeptide, a 560 aa mature extracellular domain, a 21 aa transmembrane domain and a 157 aa cytoplasmic domain. The human, mouse and rat proteins share approximately 98% homology.
Product Name:
Rat Cadherin-8/CDH8 Recombinant Protein (RPES4899)
Product Code:
RPES4899
Size:
5µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
CDH8
Accession:
NP_445845.2
Sequence:
Met1-Met621
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat CDH8 (NP_445845.2) (Met1-Met621) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 85 % as determined by SDS-PAGE
Mol Mass:
66.5 kDa
AP Mol Mass:
75-85 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
