BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism(s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).Immune CheckpointImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Rat BTLA/CD272 Recombinant Protein (RPES3140)
Product Code:
RPES3140
Size:
50µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
BTLA
Accession:
NP_998795.1
Sequence:
Ala6-Tyr183
Fusion tag:
C-Fc
Activity:
1. Measured by its binding ability in a functional ELISA.2. Immobilized rat BTLA-mFc at 10 µg/mL (100 ?L/well) can bind mouse HVEM-Fch . The EC50 of mouse EFNA5-Fc is 6.2-14.4 ng/mL.
Endotoxin:
<1.0 EU per µg protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the rat Btla (NP_998795.1) (Ala6-Tyr183) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
43.4 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
