SDC4 (Syndecan-4), also known as Syn4, is a transmembrane heparan sulfate proteoglycan that co-operates with integrins during cell-matrix interactions for the assembly of focal adhesions and actin stress fibers and in the phosphorylation of focal adhesion kinase (FAK) on Tyr397. Syndecan-4 plays roles in the formation of focal adhesions and stress fibers. The cytoplasmic domain of syndecan-4 interacts with a number of signalling and structural proteins, and both extracellular and cytoplasmic domains are necessary for regulated activation of associated transmembrane receptors. Syndecan-4/SDC4 is a heparan sulfate proteoglycan and works as a coreceptor for various growth factors. SDC4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cells (VSMCs) proliferation and vascular progenitor cells (VPCs) mobilization. Therefore, SDC4 may be a novel therapeutic target for preventing arterial restenosis after angioplasty.
Product Name:
Mouse Syndecan-4/SDC4 Recombinant Protein (RPES5042)
Product Code:
RPES5042
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
AA959608,AW108331,ryudocan,Synd4,syndecan-4,SDC4,SYND4,Ryudocan core protein
Accession:
O35988
Sequence:
Met1-Val146
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human MDK at 10 µg/ml (100 ?l/well) can bind mouse SDC4-Fc with a linear range of 0.16-1.25 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse SDC4 (O35988)(Met1-Val146) was expressed with the Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
40.5 kDa
AP Mol Mass:
33 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
