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Recombinant Mouse Prostatic Acid Phosphatase Protein (Fc Tag) (RPES8438)

SKU:
RPES8438
Size:
20μg
Tag:
C-Fc
Reactivity:
Mouse
Expression Host:
Mammalian
Accession:
Q8CE08
€289

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Recombinant Mouse Prostatic Acid Phosphatase Protein (Fc Tag)
SKU:RPES8438
Size:20μg
Tag:C-Fc
Reactivity:Mouse
Expression Host:Mammalian
Accession:Q8CE08
Sequence:Met1-Asn381aa
Calculated Molecular Weight:66.8 kDa
Observed Molecular Weight:80 kDa
Synonyms:5'-NT, 5'-nucleotidase, Acid phosphatase prostate, ACP 3, ACP3, acpP, Ecto-5'-nucleotidase, PAP, PPAP, Prostatic acid phosphatase, Prostatic acid phosphotase, Thiamine monophosphatase, TMPase
Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method
Purity:> 90% as determined by reducing SDS-PAGE.

ACPP (Acid phosphatase, prostate, also PAP and ACP3) is a 48-52 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature Human ACPP is 354 amino acids (aa) in length (aa 33-386). It contains one histidine phosphatase domain (aa 34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa 153-185, while another is transmembrane (previously called TMPase) and shows a 38 aa substitution for the C-terminal seven amino acids. Over aa 33-379, Human ACPP shares 84% aa identity with an Mouse ACPP.

Bio-activity:Not validated for activity
Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

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